Applications ≫

The NHQLive™ Label-Free and High Content Live Cell Imaging Analyzer is a fast and sensitive imaging tool for long-term detection of live cells, suitable for cell research.

In this case, human mesenchymal stem cells (hMSCs) were treated with 10mg/L interleukin-1β (IL-1β) and then continuously imaged for 25 hours to monitor cellular behavior under prolonged inflammatory conditions.

Precise regulation of the cell cycle is the basis of cell growth and division. In this case, the quantitative phase imaging of the NHQLive™ was used to observe the cell cycle progression of U2-OS cells (a human osteosarcoma cell line) treated with 50mM Blebbistatin, demonstrating the dynamic changes of each stage of the cell cycle under the action of inhibitors.

Bacterial viability is an important item in bacterial detection, helping researchers understand the physiological state of bacteria and their potential impacts. In the research on bacterial viability, intelligent quantitative phase microscopy can realize long-term dynamic monitoring of bacteria.This case demonstrates the rapid capture capability of NHQLive for bacterial movement by observing the motion of E. coli diluted to OD600=0.6 within a 2-second timeframe.

Traditional blood analysis methods still have room for improvement in speed, accuracy, and automation. This case demonstrates membrane fluctuations in normal human red blood cells (RBCs), which has the advantages of fast speed, high accuracy, and high automation. It can quickly obtain information such as the dry weight and density of red blood cells, providing a new reference for blood cell sorting.

Organoids are 3D structures cultured in vitro, which can provide experimental conditions closer to the in vivo environment than traditional 2D cell culture and have advantages in drug screening. In this case, the NHQLive™ was used to successfully monitor the state changes of organoids during continuous culture. With the help of LFAI™ (Label-free Artificial Intelligence) software, image stitching, segmentation, classification, detection, and tracking were performed.

Reproductive breeding is an important technical means in agriculture and animal husbandry. The activity of germ cells directly affects the success rate of fertilization and the quality of embryonic development. Accurate evaluation of germ cell activity is crucial for assisted reproduction and basic reproductive biology research. In this case, interference quantitative phase imaging technology and analysis methods were used to demonstrate the motility and morphological characteristics of human sperm.

Cell therapy relies on the ability of T cells to recognize and attack tumor cells in vivo, and T cell activity analysis is the key to evaluating T cell function and immune response. BJR T cell activity detection technology adopts a label-free method, which can perform non-invasive immunotherapy analysis, effectively detect T cell activity, and provide accurate support for the research and application of cell therapy.